AFM-Raman laboratory

Coupled AFM-RAMAN system based on NT-MDT Atomic Force Microscope (Fig. 1) and Renishaw Raman spectrometer (Fig. 2)

Coupled AFM-RAMAN system has been dedicated for measurements based on the following methods: Raman microspectroscopy, Atomic Force Microscopy and Tip-Enhanced Raman nanospectroscopy (TERS).

    AFM microscope parameters:

  • Microscope equipped with:
    • removable XYZ scanner of 90 x 90 x 9 μm range
    • removable XYZ short-distance scanner of 1 x 1 x 1 μm range
    • scanning laser for cantilever deflection positioning, emitting at 830 nm
    • optical preview system that provides the ability to scan a sample with a laser beam, cooperating with the optical head AFM in an "on-axis" configuration
    • AFM optical head with integrated 100x objective with a numeric aperture of 0.7
    • STM unit for optical head
    • unit for simultaneous AFM / Raman operation in liquids on an "on-axis" configuration equipped with a 60x immersion objective with NA = 1
  • The possibility of conducting measurements in air and liquids
  • The microscope enables work in air in a static mode:
    • in contact mode
    • in Lateral Force Microscopy (LFM) mode
  • The microscope enables work in air in a dynamic mode:
    • in non-contact and tapping mode
    • in phase imaging mode with simultaneous surface topography imaging
    • in force modulation mode
    • in force-distance curve measurement mode
    • in force-distance curve mapping mode with simultaneous topographic map acquisition
  • measurements in upright configuration
  • Simultaneous AFM / Raman mapping from the same sample location in an "on-axis" geometry

Fig. 1. NT-MDT Atomic Force Microscope.

    Parameters of Raman dispersive spectrometer:

  • Spectra range : 100 cm-1 – 4100 cm-1
  • Spectrometer equipped with a triple detection path
  • Excitation suorces:
    • point 532 nm laser of 50 mW input power, emitting linearly polarized light, air-cooled
    • He-Ne 633nm laser of 15 mW input power, emitting linearly polarized light, air-cooled
    • point 785 nm NIR laser of 100 mW input power, emitting linearly polarized light, air-cooled
    • Nd:YAG 1064 nm laser of 100mW input power, emitting linearly polarized light, with the plasma filter, air-cooled
  • Detectors:
    • Thermoelectrically cooled to -70°C CCD detector with matrix size of 1024 x 256 pixels, for operation in the spectral range from 200 nm to 1050 nm
    • EMCCD detector with matrix size of 1600 x 200 pixels, 90% quantum efficiency and read speed of 1500 spectra/s; thermoelectrically cooled to -100°C
    • InGaAs linear detector with a 512 x 1 pixel matrix size designed for operation in the spectral range from 0.6 to 1.7 μm, recording speed 170 spectra/s and 85% quantum efficiency, thermoelectrically cooled to -90°C
  • Diffraction gratings: 600 lines/mm, 830 lines/mm, 1200 lines/mm, 1800 lines/mm
  • Edge filters for 532, 633, 785 and 1064 nm excitation lines
  • Confocal optical microscope integrated with a Raman spectrometer and equipped with:
    • color video camera for samples observation in white light with simultaneous visualization of the laser spot
    • objectives with magnification of: 5x, 20x, 50x, 60x (immersion), 100x
    • automatically controlled XYZ scanning table enabling Raman imaging with at least 0.1 μm step size for XYZ
  • Possibility of Raman imaging/mapping using methods:
    • point
    • linear
    • streamline imaging
    • fast point imaging in high spatial resolution mode
    • ultrafast imaging: 1000 spectra/s
    • 3D imaging
  • Additional equipment:
    • CO2 incubator with temperature control for life cell imaging
    • polarizers
    • macro sampling set

Fig. 2. inVia Raman dispersive spectrometer provided by Renishaw.