Laboratory of vibrational microspectroscopy I

In Raman Spectroscopy Laboratory we have the following research areas:

  1. Study of the effect of ionizing radiation on biological systems
  2. Study of radiation damage in tumor cells induced by ionizing radiation is performed on the basis of an analysis of Raman bands in the range characteristic for these biomolecule and that are most susceptible to this type of radiation (DNA, RNA).

  3. Conformational studies of organic molecules adsorbed to the nanoparticle surface
  4. Synthesis of stable metal nanoparticles requires application of suitable stabilizers which may be various types of biomolecules such as amino acids or proteins. Due to the dependence of biological properties of the nanoparticles on the structure stabilizer adsorbed on the surface, in our study, we try to determine the conformation of these the molecules with SERS technique.

  5. Study of molecular structure selected protein kinase inhibitors and their effects in vitro on cancer cells.

  6. Conformational studies of biological molecules using methods AFM + Raman and TERS

Fig. 1 Study of radiation damage in tumor cells using Raman spectroscopy.

AFM- IR (NanoIR) method combines the atomic force microscopy (AFM) with spectroscopy in the infrared range (IR). It is a modern technique, using which it is possible to study objects smaller than it is allowed by diffraction limit for infrafred radiation. The ANASYS (USA) NanoIR2 spectrometer is the second in the world system equipped with two lasers: optical parametric oscillator (OPO) and quantum cascade laser (QCL). The spectrometer enables to study the spatial distribution of chemical compounds in the specified structures (Fig. 1 B) and spectral measurements in the infrared range (Fig. 1 C) with nanometric spatial resolving power.Currently in the laboratory, among all, studies are conducted on the effect of the chromatin structure on the formation of chromosome aberrations, the effect of ionizing radiation on the chemical structure of cells, tissues and study the conformation of DNA in a spore forms of bacteria.

Fig. 2 Chromosome study using AFM- IR method: A- topography AFM, B- nucleic acid spatial distribution (╬Żas (O-P-O) 1240 cm-1), C- IR spectrum taken from a point indicated by the arrow on chromosome.