Cytogenetics laboratory, PCR system, laboratory image analysis, Komet system

To study DNA radiation damage in our laboratory are used, among others, the following methods:

• Analysis of incidence of dicentric chromosomes using classical cytogenetic methods (CA)

Fig 1 View of the metaphase optical microscope

• Analysis of incidence of translocations using FISH (Fluorescent In Situ Hybridization) technique

Fig. 2 abeled 3 pairs of chromosomes. Colors: red (chromosome 1, Texas Red), green (chromosome 2, FITC), yellow (chromosome 4, mixture of FITC and Texas Red)

• Analysis of incidence of micronuclei in binucleated cells (Micronucleus assay )

Fig 3 Binucleated cell with one visible micronucleus

• Alkaline version of the single cell electrophoresis in agarose gel (Single cell gel electrophoresis, SCGE ), komet method (Comet assay )

Fig 4 The picture shows lymphocytes DNA trace observed after using a single cell electrophoresis in agarose gel.

• PCC analysis (Premature Chromosome Condensation ) - premature chromatin condensation in the version with CHO cells (Chinese Hamster Ovary ) and chemical induces fusion

• Analysis of gene expression using RT-PCR technique ( Real-Time Polymerase Chain Reaction)