Mechanical properties of living cancer cells and tissues.
During last two decades, mechanical properties of individual cells have been recognized and partially established as a quantitative measure of cellular deformability with potential use as a non-labelled biomarker of various pathologies, including cancer. This is possible due to technological advancements of techniques enabling to measure properties of single cells such as atomic force microscopy (AFM). Starting from the first AFM experiments by Lekka at al. in 1999 demonstrating large deformability of single cancerous cell, now, there is a large research data showing potential applications of AFM in oncology. Nevertheless, still there is a challenge to use biomechanical markers for cancer tracking and diagnosis
Comparison between non-metastatic and metastatic cell lines from different tissue types. Cancer cells are softer as compared to normal ones (Lekka et al. Micron, 2012).
Correlation between mechanical and surface properties of human bladder cancer cells studied by AFM and ToF SIMS techniques.
Beyond the doubt, the hypothesis that majority of cancer cells are softer than their normal counterpartnersseems to be well accepted and established. So far, research delivered data showing, on the onehand, the applicability of cellular mechanics as a biomarker of various pathological changes, while, on the other hand, reveal relativenature of cellular biomechanics.Our aim is to demonstrate the applicability of AFM and also to find correlations between biomechanics and structure and surface properties of cells. The example of such studies show the correlation between elasticity of human bladder cancer cells and their surface properties studied by time-of-flight secondary ion mass spectrometry (ToF SIMS).