Force-induced single molecule/cell unbinding
Thanks to the possibility of measuring the unbinding force as a function of a loading rate, it is possible to study the dissociation of an individual molecular complex. Based on that, the energy landscape and parameters such as the position of the energy barrier, dissociation rate and lifetime of the complex can be obtained. These parameters are not accessible by other methods.
Force-induced unbinding can also be applied to study the properties of single molecular complexes directly on a surface of living cells. Another possibility is to quantify adhesion forces of a single cell either to various materials or to other cells. The latter approach is called single-cell force spectroscopy. In our research, we are interested in quantification of unbinding forces of both integrins and syndecans in normal and cancer cells.
- K.Herman, A.Ptak, M.Lekka – Unbinding kinetics of syndecans by single-molecule force spectroscopy – Journal of Physical Chemistry Letters 9 (2018) 1509-1515.
- K. Herman, M. Weiss, M. Lekka, A. Ptak – How Complex Is the Concanavalin A–Carboxypeptidase Y Interaction? ACS Chem. Biol., 14 (2019) 1611-1618.
- M. Lekka, K.Herman, J.Zemła, L.Bodek, G.Pyka-Fościak, D.Gil, J.Dulińska-Litewka, A.Ptak, P.Laidler – Probing the recognition specificity of αVβ1 integrin and syndecan-4 using force spectroscopy – Micron 137 (2020) 102888.