Research tasks

Within the first year of the project realization, all tasks were realized for bladder cancer cells because they are characterized by a large deformability difference linked with the organization of the actin cytoskeleton inside the cells. Three cell lines were chosen:

  • HCV29 – non-malignant cell cancer of the ureter
  • HT1376 – bladder carcinoma
  • T24 – transitional cell cancer (bladder)
Fluorescence micrographs showing the organization of actin filaments (A-C, green) in bladder cancer cells (blue – cell nucleus, scale bar 20 microns), and the distribution of Young’s (elastic) modulus of living cells (D).

Research tasks foreseen to be realized during the first year of project realization:

  • Task 1: Microfluidic device design and production, leader NTNU
  • Task 4: Preparing surfaces selectively capturing sorted cells, leader IFJ PAN
  • Task 9: Characterization of invasive phenotype of studied cells, leader IFJ PAN
  • Task 5: Verification of biological activity of immobilized molecules, leader IFJ PAN
  • Task 2: Optimization of properties of designed microfluidic systems, leader NTNU & IFJ PAN

Research tasks foreseen to be realized during the second year of project realization:

  • Task 9: Characterization of invasive phenotype of studied cells, leader IFJ PAN
  • Task 3: Implementation of a microfluidic device for sorting and selective capturing of melanoma cells, leader NTNU
  • Task 7: Measurements of rheological properties of suspended cells, leader NTNU & IFJ PAN
  • Task 8: Development of theoretical model describing rheology of suspended cells, leader NTNU

Research tasks foreseen to be realized during the third year of project realization:

  • Task 10: Comparing mechanical properties of suspended and adherent cells, leaders IFJ PAN & NTNU
  • Task 11: Sorting and selective capturing cells in mixtures, leaders IFJ PAN & NTNU