An inverted optical microscope (Olympus IX83) is equipped with a standard fluorescence mode (DAPI, FITC, TRITC). Fluorescence is excited with a 100W mercury lamp (Olympus). The system is integrated with a high precision automatic table (Proscan). The temperature-controlled table CO2 incubator and gas exchange 3-input system (Tokai Hit) allow live-cell imaging in various conditions (normoxia, hypoxia).
